Education and training
Postdoctoral fellow, Infectious Diseases & Vaccinology, University of California, Berkeley - Laboratory of Prof. Lee W. Riley - 2008-2012
Ph.D., Infectious Diseases, School of Medicine, Lille, France- Laboratory of Prof. James McKerrow, University of California, San Francisco - 2006
Master, Cooperation and development, European School of Advanced Studies, Pavia, Italy - 2003
Master, Parasitology, Montpellier University of Science, France - 2001
B.S., Biochemistry, Lille University of Science, France/Imperial College, London, UK - 2000
My research in the lab focuses on tuberculosis. I am specifically interested in understanding what constitutes a protective host immune response capable of building a functioning granuloma to wall off Mycobacterium tuberculosis. The study aims to assess the relative contribution of monocyte subsets to the maintenance of M. tuberculosis-induced granulomas. This involves the establishment of M. tuberculosis-induced granulomas in human lung tissue models based on fibroblasts, endothelial and epithelial cells; FACS isolation and functional characterization of monocyte subsets CD14+CD16-, CD14+CD16+ and CD14dimCD16+; assessment of granuloma expansion or disruption, cell death, cytokine production, and bacterial growth; immunofluorescence staining of granulomas in lung tissues from non-human primates.
My other project involves the identification of a host signature to distinguish pulmonary TB and non-TB pulmonary diseases. This work directly addresses the fact that clinically relevant TB biomarkers need to be able to distinguish pulmonary TB and non-TB pulmonary diseases. We conducted a pilot study in collaboration with a research institute in Brazil. Hundred human samples were collected, including from patients with active TB, pneumonia or asthma. The samples were analyzed by real-time PCR and a host signature based on the expression of three genes was identified. This project is now entering a larger-scale study and involves new collaborative research with clinics and laboratories in Brazil.
Publications1. Milano M, Moraes MO, Rodenbusch R, Delcroix M, Carvalho CX, Mousquer G, Laux da Costa L, Unis G, Dalla Costa ER, Rossetti MLR. (2016) Single nucleotide polymorphisms in IL17 and IL6 are associated with decreased risk for pulmonary tuberculosis development in Southern Brazilian population. PLoS ONE 11(2): e0147814.2. Laux Da Costa L*, Delcroix M*, Dalla Costa ER, Milano M, Francis S, Unis G, Rossato Silva D, Riley LW, Rossetti MLR. (2015) *Authors contributed equally to this work. A three-gene host signature to distinguish tuberculosis from other pulmonary diseases. Tuberculosis 95(4), 421-53. Delcroix M and Riley LW. (2010) Cell-penetrating peptides for antiviral drug development. Review. Pharmaceuticals 3(3), 448-4704. de Oliveira Fraga LA, Lamb EW, Moreno EC, Chatterjee M, Dvořák J, Delcroix M, Sajid M, Caffrey CR, Davies SJ. (2010) Rapid induction of IgE responses to a worm cysteine protease during murine pre-patent schistosome infection. BMC Immunology 11:565. Dvořák J, Mashiyama ST, Sajid M, Braschi S, Delcroix M, Schneider EL, McKerrow WH, Bahgat M, Hansell E, Babbitt PC, Craik CS, McKerrow JH, and Caffrey CR. (2009) SmCL3, a gastrodermal cysteine protease of the human fluke Schistosoma mansoni. PLoS neglected tropical diseases 3(6), e4496. Delcroix M, Medzihradsy K, Caffrey CR, Fetter RD and McKerrow JH. (2007) Proteomic analysis of adult S. mansoni gut contents. Molecular and Biochemical Parasitology 154, 95-977. Delcroix M, Sajid M, Caffrey C.R., Lim K.C., Hsieh I., Bahgat M., Dissous C., and McKerrow J.H. (2006) A multienzyme network functions in intestinal protein digestion by a platyhelminth parasite. Journal of Biological Chemistry 281, 39316-393298. Dvorak J, Delcroix M, Rossi A, Vopalensky V, Pospisek M, Sedinova M, Mikes L, Sajid M, Sali A, McKerrow JH, Horak P, and Caffrey CR. (2005) Multiple cathepsin B isoforms in schistosomula of Trichobilharzia regenti: identification, characterisation and putative role in migration and nutrition. International Journal of Parasitology 35, 895-9109. Arai M, Billker O, Morris HR, Panico M, Delcroix M, Dixon D, Ley SV, and Sinden R.E. (2001) Both mosquito-derived xanthurenic acid and a host blood-derived factor regulate gametogenesis of Plasmodium in the midgut of the mosquito. Molecular and Biochemical Parasitology 116, 17-2410. Lardans V, Coppin J, Vicogne J, Aroca E, Delcroix M, and Dissous C. (2001) Characterization of an insulin receptor-related receptor in Biomphalaria glabrata embryonic cells. Biochimica et Biophysica Acta 1510, 321-329